Search results for "Selectable marker"

showing 3 items of 3 documents

Protection of HeLa‐T4 + cells against human immunodeficiency virus (HIV) infection after stable transfection with HIV LTR‐2‘,5‘‐oligoadenylate synthe…

1990

An expression vector (pU3R-III/2-5AS) of human 2',5'-oligoadenylate (2-5A) synthetase was constructed in which a cDNA encoding an active form of the enzyme was located 3' to a 3'-long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1). The LTR-directed expression of this hybrid DNA could be activated in trans by the HIV tat gene product. This vector was used for transfection of HeLa-T4+ cells, which are permissive to HIV infection, as well as of normal HeLa cells. HIV replication after infection of the CD4-receptor-bearing HeLa-T4+ cells with HIV-1 was found to be strongly reduced when drug-selected cells cotransfected with pU3R-III/2-5AS and a hygromycin B resistance gene…

Expression vector2'-5'-OligoadenylatevirusesTransfectionBiologyBiochemistryVirologyMolecular biologyVirusLong terminal repeatGene productchemistry.chemical_compoundchemistryGeneticsMolecular BiologyHygromycin BSelectable markerBiotechnologyThe FASEB Journal
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Strong immunogenic potential of a B7 retroviral expression vector: generation of HLA-B7-restricted CTL response against selectable marker genes.

1998

The stimulation of a specific immune response is an attractive goal in cancer therapy. Gene transfer of co-stimulatory molecules and/or cytokine genes into tumor cells and the injection of these genetically modified cells leads to tumor rejection by syngeneic hosts and the induction of tumor immunity. However, the development of host immune response could be either due to the introduced immunomodulatory genes or due to vector components. In this study, human renal cell carcinoma cell lines were modified by a retrovirus to express the co-stimulatory molecule B7-1 together with the hygromycin/thymidine kinase fusion protein (HygTk) as positive and negative selection markers. These B7-1-transd…

Genetic MarkersT cellGenetic VectorsLymphocyte ActivationHLA-B7 AntigenImmune systemRetrovirusAntigens NeoplasmGeneticsmedicineTumor Cells CulturedHumansMolecular BiologyCarcinoma Renal CellSelectable markerExpression vectorbiologyHistocompatibility Antigens Class IGene Transfer TechniquesGenetic TherapyAcquired immune systembiology.organism_classificationMolecular biologyKidney Neoplasmsmedicine.anatomical_structureRetroviridaeCell cultureThymidine kinaseCancer researchB7-1 AntigenMolecular MedicineT-Lymphocytes CytotoxicHuman gene therapy
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Yeast vectors for the integration/expression of any sequence at theTYR1 locus

2007

We have constructed new yeast vectors for targeted integration and conditional expression of any sequence at the Saccharomyces cerevisiae TYR1 locus which becomes disrupted. We show that vector integration is not neutral, causing prototrophy for tyrosine and auxotrophy for the vector's selectable marker (uracil or leucine, depending on the vector used). This feature allows a double screening of transformed yeast cells, improving the identification of colonies with the desired chromosomal structure. The GAL10 gene promoter has been added to drive conditional expression of cloned sequences. Using these vectors, chromosomal structure verification of recombinant clones is no longer necessary, s…

Sequence analysisAuxotrophyGenetic VectorsGreen Fluorescent ProteinsMolecular Sequence DataSaccharomyces cerevisiaeBioengineeringLocus (genetics)Saccharomyces cerevisiaeBiologyPolymerase Chain ReactionApplied Microbiology and BiotechnologyBiochemistryGenes ReporterGene Expression Regulation FungalGeneticsDNA FungalSelectable markerRegulation of gene expressionGeneticsExpression vectorBase SequenceSequence Analysis DNAbiology.organism_classificationMutagenesis InsertionalTyrosineHeterologous expressionBiotechnologyYeast
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